Overall, we propose SPRY2, PTEN, and PP2A status as an important determinant of prostate cancer progression. Note: western blots are semi quantitative so. Popular Answers (1) 1) Open western image in Image J, select Rectangular Selections tool from the ImageJ toolbar and select first western band. This underlines the cooperation between SPRY2 deficiency and PTEN or PP2A inactivation in promoting tumorigenesis. quantification of protein extracts prior to western blotting is crucial first step in generation of accurate data. gel analysis function such as analysing intensity of western blot bands. Furthermore, loss of SPRY2 expression correlates strongly with loss of PTEN and/or PP2A subunits in human prostate cancer. The ImageJ is a java-based public-domain image processing and analysis programme. Together, these results show that loss of Pten cooperates with Spry2 deficiency by bypassing a novel tumor suppressor checkpoint.
#Imagej western blot quantification software
and western blot quantification (by software programs such as ImageJ). In murine prostate cancer models, Pten haploinsufficiency synergized with Spry2 deficiency to drive tumorigenesis, including metastasis. Western blot loading control antibodies, actin, tubulin, vinculin, GAPDH, PCNA. Mechanistically, the PP2A-mediated growth arrest depends on GSK3β and is ultimately mediated by nuclear PTEN. then assayed for whole protein content via protein quantification assay (ex. Quantification of microglia soma was completed using Fiji (Image J) Analyse. Western blot protocols share the similar steps of other blotting protocols. In addition to AKT and ERK activation, SPRY2 loss also activates a PP2A-dependent tumor suppressor checkpoint. The western blots were captured with a digital camera, and the intensities. Here, we show that SPRY2 deficiency alone triggers activation of AKT and ERK, but this is insufficient to drive tumorigenesis. The first is to treat each row as a horizontal lane and use ImageJs gel analysis function. The molecular basis of cooperation between these genetic alterations is unknown. There are two built in methods for analyzing a dot blot in ImageJ. The negative regulators of these pathways, including sprouty2 (SPRY2), protein phosphatase 2A (PP2A), and phosphatase and tensin homolog (PTEN), are commonly inactivated in prostate cancer. Concurrent activation of RAS/ERK and PI3K/AKT pathways is implicated in prostate cancer progression.
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